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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Chronic Voluntary Alcohol Consumption Alters Promoter Methylation and Expression of Fgf-2 and Fgfr1
doi: 10.3390/ijms24043336
Figure Lengend Snippet: Alterations in fibroblast growth factor receptor 1 ( Fgfr1) methylation after alcohol consumption. ( a ) Schematic representation of significant changes in the methylation pattern of the murine Fgfr1 promoter. Chronic alcohol consumption led to hypermethylation of CpG −361 in dorsolateral striatum (DLS) and CpG −270 in prefrontal cortex (PFC). We used bisulfite conversion followed by direct capillary sequencing and two-tailed t -tests to identify the significantly mapped different CpG positions (total = 2). Arrowheads indicate significant alterations ( p ≤ 0.05) in methylation rate (black = hypermethylation in the alcohol group compared with water-drinking control mice). ( b ) Estimated marginal means (EMM) of the methylation rate of all seven examined CpG sites in the Fgfr1 promoter in PFC and DLS of male mice after chronic alcohol exposure. Data are represented as mean ± SEM (n = 10 biological replicates) with a two-tailed Student’s t -test, * p ≤ 0.05; ** p ≤ 0.01 compared with the control group.
Article Snippet: Primer sequences were designed using the Geneious R11 software (Biomatters Ltd., Auckland, New Zealand) to cover CpG-sites located immediately upstream and within exon 1 of the Fgf-2 or
Techniques: Methylation, Sequencing, Two Tailed Test
Journal: International Journal of Molecular Sciences
Article Title: Chronic Voluntary Alcohol Consumption Alters Promoter Methylation and Expression of Fgf-2 and Fgfr1
doi: 10.3390/ijms24043336
Figure Lengend Snippet: Expression and unified analysis of Fgfr1 . Expression profiles of the Fgfr1 gene in six murine brain areas. Fgfr1 expression was significantly decreased in the dorsomedial striatum (DMS) of mice consuming 20% alcohol for a six-week period compared with the control mice. Data are presented as mean ± SEM with squares depicted as individual data points (n = 7–10 biological replicates) analyzed with Student’s paired t -test, *** p ≤ 0.001.
Article Snippet: Primer sequences were designed using the Geneious R11 software (Biomatters Ltd., Auckland, New Zealand) to cover CpG-sites located immediately upstream and within exon 1 of the Fgf-2 or
Techniques: Expressing